CN
High Throughput Screening

Identifying New Chemical Matter via High Throughput Screening (HTS)

 

State-of-the-art Equipment for HTS Support

  • Automation system
    • - Tecan
    • - POD and Echo
    • - JANUS
    • - BRAVO
    • - Platemate Plus
  • Plate washers
    • - Elx405 and Elx406
    • - DropArray Washing StationPlate Sealers
  • Readers to support HTS
    • - BD Biosciences Flow Cytometer with HTS mode
    • - Envision (with stacker)
    • - EnSpire2300
    • - FlexStation III
    • - Bioplex 200 Luminex
    • - FLIPR and Hamammatsu
    • - Topcount and Microbeta2
    • - Q-Patch and Ion Works
    • - Acumen and Odyssey (Li-COR)

 

HTS Compound Collection

  • Majority of the collection is semi-exclusive, non-commercially available
  • Selected from >550K compounds based on diversity, properties, PAINS* & Vacca-Reos# structural filters, reviewed by medicinal chemists, and Rule-of-5 Multi Parameter Optimization (MPO)
  • Total 167k compounds were collected and another 50k compounds expansion in progress
  • Arrayed in 384 plate ready for HTS
  • Stocks and powders available in China

 

HTS Collection (166K) - Property Distributions

 

WuXi HTS Case Study

  • Over 40 HTS successfully completed with WuXi HTS library.
  • Multiple HTS projects have successfully identified hits for further lead identification and optimization.
  • Targets cover divers fields
    • - Epigenetic targets
    • - GPCRs and nuclear receptors
    • - Kinases
  • Example of primary screening QC data
    • - Z’ value of plates in primary screening
    • - IC50 result of reference compound in cherry picking screening

 

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